Difference between revisions of "Part:BBa K1500014:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | mutagenized mutH was ligated into the paroF+mCherry-LVA construct plasmid | |
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===Source=== | ===Source=== | ||
Latest revision as of 23:20, 17 October 2014
paroF->MCherry-MutH(E56A)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1346
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
mutagenized mutH was ligated into the paroF+mCherry-LVA construct plasmid
Source
paroF, mutH are genomic XL1Blue, mCherry is from distribution kit.