Difference between revisions of "Part:BBa K1465102"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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Fumarate reductase is part of the anaerobic fumarate respiration in <i>E. coli</i>. It catalyzes the reaction from fumarate into succinate using fumarate as a final electron acceptor in anaerobic fumarate respiration. The related enzyme in aerobic respiration is the succinate dehydrogenase, which catalyses the reaction from succinate to fumarate. | Fumarate reductase is part of the anaerobic fumarate respiration in <i>E. coli</i>. It catalyzes the reaction from fumarate into succinate using fumarate as a final electron acceptor in anaerobic fumarate respiration. The related enzyme in aerobic respiration is the succinate dehydrogenase, which catalyses the reaction from succinate to fumarate. | ||
The electrons are transferred from succinate to FAD<sup>+</sup> producing fumarate and FADH<sub>2</sub>. The succinate dehydrogenase is also a membrane enzyme and it is part of the citric acid cycle. They both belong to the respiration complex II. Naturally there is no activity of both enzymes at the same time in <i>E. coli</i> cells. (<a href="#Iverson1999">Iverson <i>et al.</i>, 1999</a>)<br> | The electrons are transferred from succinate to FAD<sup>+</sup> producing fumarate and FADH<sub>2</sub>. The succinate dehydrogenase is also a membrane enzyme and it is part of the citric acid cycle. They both belong to the respiration complex II. Naturally there is no activity of both enzymes at the same time in <i>E. coli</i> cells. (<a href="#Iverson1999">Iverson <i>et al.</i>, 1999</a>)<br> | ||
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To conclude the mediator-dependent activity of the fumarate reductase could serve as an energy source for bacterial cells. <br> | To conclude the mediator-dependent activity of the fumarate reductase could serve as an energy source for bacterial cells. <br> | ||
We plan on comparing the activity of the fumarate reductases (Frd) from <i>Escherichia coli</i> KRX and the fumarate reductase (Fum)from <i>Actinobacillus succinogenes</i> working with different mediators, for example neutral red or bromphenol blue, as electron donors. | We plan on comparing the activity of the fumarate reductases (Frd) from <i>Escherichia coli</i> KRX and the fumarate reductase (Fum)from <i>Actinobacillus succinogenes</i> working with different mediators, for example neutral red or bromphenol blue, as electron donors. | ||
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Revision as of 23:20, 17 October 2014
Fumarate reductase (frd) from E. coli under the control of the T7 promoter
Fumerate reductase (frd) from E. coli under the control of the T7 promoter
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1352
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 666
Illegal AgeI site found at 741
Illegal AgeI site found at 3023 - 1000COMPATIBLE WITH RFC[1000]