Difference between revisions of "Part:BBa K1316020"
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Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgA is the major curli subunit. | Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgA is the major curli subunit. | ||
A His tag was added at the C-terminus of csgA's coding sequence. This His has two functions: on one hand it allows easy purification of the CsgA protein; on the other hand, it enables the formation of conductive curli by adding gold nanoparticles (AuNP) with Nickel-Nitrilotriacetic-acid (NTA-Ni) attached to them. This last part containing Nickel would bind to the Histidines of the curli fibril, whereas the gold attached to it will make the environment more conductive. | A His tag was added at the C-terminus of csgA's coding sequence. This His has two functions: on one hand it allows easy purification of the CsgA protein; on the other hand, it enables the formation of conductive curli by adding gold nanoparticles (AuNP) with Nickel-Nitrilotriacetic-acid (NTA-Ni) attached to them. This last part containing Nickel would bind to the Histidines of the curli fibril, whereas the gold attached to it will make the environment more conductive. | ||
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| + | <h3> Characterisation </h3> | ||
| + | This part was made to construct BioBricks BBa_K1316013 and BBa_K1316014. For more information about the characterisation of this construct visit the pages of these BioBricks or the Conductive curli module characterisation on our wiki page (http://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation)! | ||
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Latest revision as of 21:59, 17 October 2014
2nd part of Constitutive Anderson promoter + csgA:Histag curli gene
Curli are extracellular amyloids that form fibers. They are involved in adhesion, cell aggregation and biofilm formation. CsgA is the major curli subunit. A His tag was added at the C-terminus of csgA's coding sequence. This His has two functions: on one hand it allows easy purification of the CsgA protein; on the other hand, it enables the formation of conductive curli by adding gold nanoparticles (AuNP) with Nickel-Nitrilotriacetic-acid (NTA-Ni) attached to them. This last part containing Nickel would bind to the Histidines of the curli fibril, whereas the gold attached to it will make the environment more conductive.
Characterisation
This part was made to construct BioBricks BBa_K1316013 and BBa_K1316014. For more information about the characterisation of this construct visit the pages of these BioBricks or the Conductive curli module characterisation on our wiki page (http://2014.igem.org/Team:TU_Delft-Leiden/Project/Life_science/curli/characterisation)!
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 8
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]