Difference between revisions of "Part:BBa K1362023"

Latest revision as of 21:54, 17 October 2014

RBS + Xylanase (with His6)

Xylanases are enzymes that degrad Xylan, a major component of wood. This is a modified version of BBa_K1175005 that is from B. subtilis (XylA). To ensure that it is kept intracellular we removed a signaling peptide from the N-terminus. Furthermore we added a His-Tag to simplify purification. Xylanases are interesting enzymes for industry as they have a huge variety of applications.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 22
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 426
Illegal SapI.rc site found at 480

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1362023 short</partinfo>
-???
+Xylanases are enzymes that degrad Xylan, a major component of wood. This is a modified version of <partinfo>BBa_K1175005</partinfo> that is from ''B. subtilis'' (XylA). To ensure that it is kept intracellular we removed a signaling peptide from the N-terminus. Furthermore we added a His-Tag to simplify purification. Xylanases are interesting enzymes for industry as they have a huge variety of applications.
 <!-- Add more about the biology of this part here