Difference between revisions of "Part:BBa K1400003"
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Figure 1: Characterization of pTRE via dual drug induction. pTRE has 4 activating tetr sites and not repressing sites, so increasing aTC increases activation. Estradiol has no effect beyond auto-fluorescence. Fluorescence values were give arbitrarily by the flow cytometer software.
  
 
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Revision as of 21:52, 17 October 2014

pTre(4) Single input tet responsive promoter

An improvement upon the promoter in BBa_K1164007. A single tet responsive promoter created by removing the 2 most distal (from TATA box) gal4 sites, and replacing the last 2 gal4 sites in the gal promoter with tet sites. The two mig1 sites were replaced as well. It was designed to have similar expression to our modified gal promoter (BBA_K1400002).

Figure 1: Characterization of pTRE via dual drug induction. pTRE has 4 activating tetr sites and not repressing sites, so increasing aTC increases activation. Estradiol has no effect beyond auto-fluorescence. Fluorescence values were give arbitrarily by the flow cytometer software.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 35
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 166