Difference between revisions of "Part:BBa K1362020"
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<partinfo>BBa_K1362020 short</partinfo>
 
<partinfo>BBa_K1362020 short</partinfo>
  
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Xylanases are enzymes that degrad Xylan, a major component of wood. This is a modified version of <partinfo>BBa_K1175005</partinfo> that is from ''B. subtilis''.
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Xylanases are enzymes that degrad Xylan, a major component of wood. This is a modified version of <partinfo>BBa_K1175005</partinfo> that is from ''B. subtilis'' (XylA). To ensure that it is kept intracellular we removed a signaling peptide from the N-terminus. Xylanases are interesting enzymes for industry as they have a huge variety of applications.
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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To characterize our part in respect to its thermal stability we conducted a Heatshock Assay. Firstly the Xylanases were incubated at 37 °C, 50 °C, 56 °C, 60 °C and 66 °C. After 30 minutes substrate (EnzChek® Ultra Xylanase Assay Kit) was added and the fluorescence was measured for 150 minutes in a platereader at 37 °C. The results indicate a reduction of activity at temperatures higher than 50 °C and complete loss of function for temperatures higher than 60 °C.
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[[File:Heatshockassay_linear_nonpurified_xylanas.png|300px|thumb|left|Activity after Heatshock assay for 30 minutes of linear Xylanase]]
  
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K1362020 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1362020 SequenceAndFeatures</partinfo>

Revision as of 21:32, 17 October 2014

RBS + Xylanase

Xylanases are enzymes that degrad Xylan, a major component of wood. This is a modified version of BBa_K1175005 that is from B. subtilis (XylA). To ensure that it is kept intracellular we removed a signaling peptide from the N-terminus. Xylanases are interesting enzymes for industry as they have a huge variety of applications.


Usage and Biology

To characterize our part in respect to its thermal stability we conducted a Heatshock Assay. Firstly the Xylanases were incubated at 37 °C, 50 °C, 56 °C, 60 °C and 66 °C. After 30 minutes substrate (EnzChek® Ultra Xylanase Assay Kit) was added and the fluorescence was measured for 150 minutes in a platereader at 37 °C. The results indicate a reduction of activity at temperatures higher than 50 °C and complete loss of function for temperatures higher than 60 °C.

Activity after Heatshock assay for 30 minutes of linear Xylanase

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 22
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 426
    Illegal SapI.rc site found at 480