Difference between revisions of "Part:BBa K1453006"
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<partinfo>BBa_K1453006 short</partinfo> | <partinfo>BBa_K1453006 short</partinfo> | ||
| − | This part is designed as a scaffold of the second kind of connectee, which can | + | This part is designed as a scaffold of the second kind of connectee, which can be free in cytoplasm. |
== Structure == | == Structure == | ||
Latest revision as of 21:31, 17 October 2014
TAL USB-His Tag
This part is designed as a scaffold of the second kind of connectee, which can be free in cytoplasm.
Structure
Figure 1 TAL USB-His Tag
In order to bind TAL protein designed by 2012 Freiburg iGEM team, the TAL USB also consists of T1 sequence, T14 sequence and two sites for type II restriction enzyme BsmBI.
When digested with BsmBI, this part can produce two sticky-ends that can bind TAL-Protein DiRepeat (Bba_K747000 to Bba_K747095)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]