Difference between revisions of "Part:BBa K1316002"
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<partinfo>BBa_K1316002 short</partinfo>
 
<partinfo>BBa_K1316002 short</partinfo>
  
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Promoter of the ''yqjF'' gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB[1].
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Promoter of the ''yqjF'' gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.[1]
  
  

Revision as of 21:27, 17 October 2014

yqjF promoter

Promoter of the yqjF gene, which is activated by the presence of several nitrogen-based compounds such as 2,4,6-TNT, 2,4-DNT and 1,3-DNB.[1]


Characterisation

The functionality of the yqjF promoter is proven by the production of mKate2 in response to 2,4-DNT on the characterisation of BioBricks BBa_K1316007 (plate reader experiment) and BBa_K1316003 (FACS experiment).


References

[1] S. Yagur-Kroll, S. Belkin et al., “Escherichia Coli bioreporters for the detection of 2,4-dinitrotoluene and 2,4,6-trinitrotoluene”, Appl. Microbiol. Biotechnol. 98, 885-895, 2014.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 128
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]