Difference between revisions of "Part:BBa K1453409"

Latest revision as of 21:27, 17 October 2014

poxB-TAL1-His Tag

This part is used in our application test free in the cytoplasm. See more details please view iGEM14_SJTU_BioX_Shanghai.

Structure

Figure 1 poxB-TAL1-His Tag

This part is based on the BBa_1453006 and BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate dehydrogenase (quinone) [EC:1.2.5.1] or poxB

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 2239
Illegal BsaI.rc site found at 2851

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1453409 short</partinfo>
 This part is used in our application test free in the cytoplasm. See more details please view iGEM14_SJTU_BioX_Shanghai.
+== Structure ==
+https://static.igem.org/mediawiki/2014/7/7e/3409.png
+'''Figure 1 poxB-TAL1-His Tag'''
 This part is based on the BBa_1453006 and BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate dehydrogenase (quinone) [EC:1.2.5.1] or poxB