Difference between revisions of "Part:BBa K1453408"

Latest revision as of 21:17, 17 October 2014

pykF-TAL1-His Tag

This part is used in our application test free in the cytoplasm. See more details please view iGEM14_SJTU_BioX_Shanghai.

Structure

Figure 1 pykF-TAL1-His Tag

This part is based on the BBa_1453006 and BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1312
12
INCOMPATIBLE WITH RFC[12]
Illegal PstI site found at 1312
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1027
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1312
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1312
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 1933
Illegal BsaI.rc site found at 2545

@@ Line 11: / Line 11: @@
 '''Figure 1 pykF-TAL1-His Tag'''
-This part is based on the BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.
+This part is based on the BBa_1453006 and BBa_K1453406 or BBa_K1453407 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.
 <!-- Add more about the biology of this part here