Difference between revisions of "Part:BBa K1372001"

Revision as of 20:59, 17 October 2014

Salicylate Inducible Suppressing System

This BB is the result of the assembly of two BB:

- BBa_K1372000:

- BBa_B0015: Double terminator consisting of BBa_B0010 and BBa_B0012.

Thus, there will be a terminator of the transcription of tRNA-SUPD gene (missing in BBa_K1372000).

This BB could thus be used as control system of the production of tRNA-SUPD depending on the concentration of a substrate (salicylate) gradually consumed by the organisms in the growth-medium. Consequentially, the production of tRNA-SupD will gradually peter out.

An example of use of this control system is described there: [http://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Salicylate Inducible System]

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1309
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1312

Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]

Revision as of 20:58, 17 October 2014 (view source) Maher (Talk \| contribs) ← Older edit		Revision as of 20:59, 17 October 2014 (view source) Maher (Talk \| contribs) Newer edit →
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	This BB could thus be used as control system of the production of tRNA-SUPD depending on the concentration of a substrate (salicylate) gradually consumed by the organisms in the growth-medium. Consequentially, the production of tRNA-SupD will gradually peter out.		This BB could thus be used as control system of the production of tRNA-SUPD depending on the concentration of a substrate (salicylate) gradually consumed by the organisms in the growth-medium. Consequentially, the production of tRNA-SupD will gradually peter out.

−	An example of use of this control system is described there: ~~lien wiki~~.	+	An example of use of this control system is described there: [http://2014.igem.org/Team:Paris_Saclay/Project/Salicylate_Inducible_System Salicylate Inducible System]