Difference between revisions of "Part:BBa K1372001"
| Line 4: | Line 4: | ||
This BB is the result of the assembly of two BB: | This BB is the result of the assembly of two BB: | ||
| − | - BBa_K1372000 | + | - [https://parts.igem.org/Part:BBa_K1372000 BBa_K1372000]: |
[[File:Paris Saclay BB2000.png|center]] | [[File:Paris Saclay BB2000.png|center]] | ||
| − | - BBa_B0015 | + | - [https://parts.igem.org/Part:BBa_B0015 BBa_B0015]: Double terminator consisting of BBa_B0010 and BBa_B0012. |
Thus, there will be a terminator of the transcription of tRNA-SUPD gene (missing in BBa_K1372000). | Thus, there will be a terminator of the transcription of tRNA-SUPD gene (missing in BBa_K1372000). | ||
| Line 31: | Line 31: | ||
<partinfo>BBa_K1372001 parameters</partinfo> | <partinfo>BBa_K1372001 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
| + | |||
| + | Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay] | ||
Revision as of 20:58, 17 October 2014
Salicylate Inducible Suppressing System
This BB is the result of the assembly of two BB:
- BBa_K1372000:
- BBa_B0015: Double terminator consisting of BBa_B0010 and BBa_B0012.
Thus, there will be a terminator of the transcription of tRNA-SUPD gene (missing in BBa_K1372000).
This BB could thus be used as control system of the production of tRNA-SUPD depending on the concentration of a substrate (salicylate) gradually consumed by the organisms in the growth-medium. Consequentially, the production of tRNA-SupD will gradually peter out.
An example of use of this control system is described there: lien wiki.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 786
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1309 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1312
Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]