Difference between revisions of "Part:BBa K1372000"

Revision as of 20:54, 17 October 2014

salicylate inducible suppressing system

This BB is the result of the assembly of two BB:

- BBa_J61051 for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).

- BBa_K228001: for the transcription of SupD-tRNA.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 786
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 77
Illegal NgoMIV site found at 618
Illegal AgeI site found at 1309
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1312

Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]

@@ Line 4: / Line 4: @@
 This BB is the result of the assembly of two BB:
-- BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).
+- [https://parts.igem.org/Part:BBa_J61051 BBa_J61051] for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).
-- BBa_K228001(lien): for the transcription of SupD-tRNA.
+- [https://parts.igem.org/Part:BBa_K228001 BBa_K228001]: for the transcription of SupD-tRNA.
 [[File:Paris Saclay BB2000.png|center]]
@@ Line 22: / Line 22: @@
 <partinfo>BBa_K1372000 parameters</partinfo>
 <!-- -->
+Made by [http://2014.igem.org/Team:Paris_Saclay iGEM Paris Saclay]