Difference between revisions of "Part:BBa K1351041"

Latest revision as of 20:52, 17 October 2014

spaBTC (Modification&Export of subtilin)

The genes spaBTC play a role for the posttranslational modifications and the transport of subtilin. spaB encodes a hydratase, spaC a cyclase, which is responsible for the formation of the thither ring. spaT is coding for a NisT-type ABC-transporter, which exports subtilin, which is later on processed by an unspecific protease (cleavage of leader sequence)

This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 2391
Illegal BamHI site found at 4661
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 2205
Illegal AgeI site found at 3213
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 257
Illegal SapI.rc site found at 2305
Illegal SapI.rc site found at 4815
Illegal SapI.rc site found at 6017

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1351041 short</partinfo>
 The genes spaBTC play a role for the posttranslational modifications and the transport of subtilin. spaB encodes a hydratase, spaC a cyclase, which is responsible for the formation of the thither ring. spaT is coding for a NisT-type ABC-transporter, which exports subtilin, which is later on processed by an unspecific protease (cleavage of leader sequence)
+This part is used in the 2014 LMU-Munich iGEM project [http://2014.igem.org/Team:LMU-Munich BaKillus].
 <!-- Add more about the biology of this part here