Difference between revisions of "Part:BBa K1334020"
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We transform this part which is constructed in the pSB1C3 backbone into the E.coli strain DH5a. After exogenous AHL (3OC6HSL ,final concentration:100nM)stimulation, we detect the relative fluorescence intensity of RFP. The following figures shows that the lux system works well. | We transform this part which is constructed in the pSB1C3 backbone into the E.coli strain DH5a. After exogenous AHL (3OC6HSL ,final concentration:100nM)stimulation, we detect the relative fluorescence intensity of RFP. The following figures shows that the lux system works well. | ||
| − | [[File:Figure 11 The function test of our part BBa_K1334020 in which the relative fluorescence reflect that the device works.png]] | + | [[File:Figure 11 The function test of our part BBa_K1334020 in which the relative fluorescence reflect that the device works.png|600px]] |
Figure 1 The function test of our part BBa_K1334020 in which the relative fluorescence reflect that the device works well. | Figure 1 The function test of our part BBa_K1334020 in which the relative fluorescence reflect that the device works well. | ||
| − | [[File:Figure 12 A picture which shows the color change of the bacteria after AHL (3OC6HSL) stimulation2.png| | + | [[File:Figure 12 A picture which shows the color change of the bacteria after AHL (3OC6HSL) stimulation2.png|400px]] |
Figure 2 A picture which shows the color change after AHL (3OC6HSL) stimulation. | Figure 2 A picture which shows the color change after AHL (3OC6HSL) stimulation. | ||
Revision as of 20:49, 17 October 2014
a signal complex to test whether the lux system works
When luxR is activated,the promoter plux will be activated,the expression of RFP is up_regulated.this device is used to test the function and sensitivity of the lux system
Usage and Biology
We transform this part which is constructed in the pSB1C3 backbone into the E.coli strain DH5a. After exogenous AHL (3OC6HSL ,final concentration:100nM)stimulation, we detect the relative fluorescence intensity of RFP. The following figures shows that the lux system works well.
Figure 1 The function test of our part BBa_K1334020 in which the relative fluorescence reflect that the device works well.
_stimulation2.png)
Figure 2 A picture which shows the color change after AHL (3OC6HSL) stimulation.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1686
Illegal AgeI site found at 1798 - 1000COMPATIBLE WITH RFC[1000]