Difference between revisions of "Part:BBa K1381017:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1381017=== | ===Applications of BBa_K1381017=== | ||
| − | + | '''The Killing system'''<br> | |
| + | We, the Uppsala iGEM Team 2014, designed the killing system to secret the colicin Fy with the help of a secretion tag, the USP45 [https://parts.igem.org/Part:BBa_K1381018 BBa_K1381018]. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice to it. This part contains the restriction sites NgoMIV at the start of the sequence to more easily fuse proteins like an export tag to it. Since it got a NgoMIV site at the start of the sequence it is possible to fuse other sequences if they follow the Freiburg Biobrick standard 25 and contains an AgeI site. However our part is not entirely built in the Freiburg standard Biobrick 25 since it does not contain an AgeI site. Read more about the system in our wiki: http://2014.igem.org/Team:Uppsala/Project_Killing | ||
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1381017 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1381017 StartReviews</partinfo> | ||
Revision as of 20:24, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1381017
The Killing system
We, the Uppsala iGEM Team 2014, designed the killing system to secret the colicin Fy with the help of a secretion tag, the USP45 BBa_K1381018. This part will be without promoter, RBS and export tag. It only contains the colicin Fy and a 6x His-tag for characterization. It is designed so that you can easily couple another promoter or export tag of your choice to it. This part contains the restriction sites NgoMIV at the start of the sequence to more easily fuse proteins like an export tag to it. Since it got a NgoMIV site at the start of the sequence it is possible to fuse other sequences if they follow the Freiburg Biobrick standard 25 and contains an AgeI site. However our part is not entirely built in the Freiburg standard Biobrick 25 since it does not contain an AgeI site. Read more about the system in our wiki: http://2014.igem.org/Team:Uppsala/Project_Killing
User Reviews
UNIQdb7acb211bb23162-partinfo-00000000-QINU UNIQdb7acb211bb23162-partinfo-00000001-QINU