Difference between revisions of "Part:BBa K1453404"
FlyFreedom (Talk | contribs) |
FlyFreedom (Talk | contribs) |
||
Line 1: | Line 1: | ||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K1453404 short</partinfo> | <partinfo>BBa_K1453404 short</partinfo> | ||
+ | |||
This part is used in our application test. See more details please search these two parts of iGEM14_SJTU_BioX_Shanghai. | This part is used in our application test. See more details please search these two parts of iGEM14_SJTU_BioX_Shanghai. | ||
Line 8: | Line 9: | ||
'''Figure 1 ssDsbA-Lgt-pykF-TAL1-His Tag''' | '''Figure 1 ssDsbA-Lgt-pykF-TAL1-His Tag''' | ||
+ | |||
This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. | This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. | ||
The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF. | The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF. |
Revision as of 19:52, 17 October 2014
ssDsbA-Lgt-pykF-TAL1-His Tag
This part is used in our application test. See more details please search these two parts of iGEM14_SJTU_BioX_Shanghai.
Structure
Figure 1 ssDsbA-Lgt-pykF-TAL1-His Tag
This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2347
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2347
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2062
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2347
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2347
Illegal AgeI site found at 862 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 483
Illegal BsaI.rc site found at 2968
Illegal BsaI.rc site found at 3580