Difference between revisions of "Part:BBa K1453404"
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'''Figure 1 ssDsbA-Lgt-pykF-TAL1-His Tag'''
 
'''Figure 1 ssDsbA-Lgt-pykF-TAL1-His Tag'''
 
This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.
 
This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.

Revision as of 19:50, 17 October 2014

ssDsbA-Lgt-pykF-TAL1-His Tag


Structure

3404.png

Figure 1 ssDsbA-Lgt-pykF-TAL1-His Tag This part is based on the BBa_K1453402 or BBa_K1453403 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089. The enzyme we used here is the pyruvate kinase (EC:2.7.1.40) or pykF.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2347
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 2062
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2347
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2347
    Illegal AgeI site found at 862
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 483
    Illegal BsaI.rc site found at 2968
    Illegal BsaI.rc site found at 3580