Difference between revisions of "Part:BBa K1462290"

 
 
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<partinfo>BBa_K1462290 short</partinfo>
 
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This part express the fourth enzyme ---- butyryl-CoA dehydrogenase. Ccr is expressed under control of GAL1 promoter and ADH1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462270, BBa_K1462280, BBa_K1462260 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway.
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[[File:Pathway.png|200px|left|frame|Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.]]
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 19:45, 17 October 2014

Gal1+cox6+Ccr+ADH1

This part express the fourth enzyme ---- butyryl-CoA dehydrogenase. Ccr is expressed under control of GAL1 promoter and ADH1 terminator. With the help of CoxVI,the enzyme will be transported into mitochondrial matrix after being translated.Together with BBa_K1462270, BBa_K1462280, BBa_K1462260 and BBa_K1462300, this part is components of the n-butanol biosynthesis pathway.

Figure 1. The n-butanol biosynthetic pathway. Enzymes are from these organisms: Erg10, S. cerevisiae; Hbd, Crt, AdhE2, Clostridium beijerinckii; Ccr, Streptomyces collinus.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1329