Difference between revisions of "Part:BBa K1462190"

 
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<partinfo>BBa_K1462190 short</partinfo>
 
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This part is constructed as a control group to BBa_K1462110 to identify Ccr has been directed to the mitochondria. The GFP is fused at the C-terminus of the enzyme to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
  
 
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Revision as of 19:19, 17 October 2014

Gal1+cox6+Ccr+GFP+ADH1

This part is constructed as a control group to BBa_K1462110 to identify Ccr has been directed to the mitochondria. The GFP is fused at the C-terminus of the enzyme to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 150
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1329
    Illegal BsaI.rc site found at 2675