Difference between revisions of "Part:BBa K1510011:Experience"
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This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | This experience page is provided so that any user may enter their experience using this part.<BR>Please enter | ||
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===Applications of BBa_K1510011=== | ===Applications of BBa_K1510011=== | ||
| − | + | Adding endolysin specific to oral bactria, Streptococcus Mutans (S.Mutans), this part can be applicated in oral hygiene technique. | |
===User Reviews=== | ===User Reviews=== | ||
<!-- DON'T DELETE --><partinfo>BBa_K1510011 StartReviews</partinfo> | <!-- DON'T DELETE --><partinfo>BBa_K1510011 StartReviews</partinfo> | ||
| + | <p> | ||
| + | By doing disc plate assay of endolysin together with several other controls, we can see that there is a clear space besides the disc of our different concentration of our endolysin supernatant. | ||
| + | </p> | ||
| + | <p> | ||
| + | As shown in the figure, ampicillin showed a significant inhibition zone as a positive control. | ||
| + | Three positive control all show there ability to kill Streptococcus Mutans by the clear zone around the disc. | ||
| + | The BHI negative control showed no killing ability as expected. | ||
| + | However, there are also little Streptococcus Mutans growing near J23100+yebF+B0015 culture supernatant. By observing the pattern on the plate, we speculated that it might be the result of the diffusion of Ampicillin beside it. | ||
| + | </p> | ||
| + | [[File:NYMU chienyunghuang endolysin function test.jpg]] | ||
<!-- Template for a user review | <!-- Template for a user review | ||
{|width='80%' style='border:1px solid gray' | {|width='80%' style='border:1px solid gray' | ||
Latest revision as of 19:14, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1510011
Adding endolysin specific to oral bactria, Streptococcus Mutans (S.Mutans), this part can be applicated in oral hygiene technique.
User Reviews
UNIQa6064944afe08238-partinfo-00000000-QINU
By doing disc plate assay of endolysin together with several other controls, we can see that there is a clear space besides the disc of our different concentration of our endolysin supernatant.
As shown in the figure, ampicillin showed a significant inhibition zone as a positive control. Three positive control all show there ability to kill Streptococcus Mutans by the clear zone around the disc. The BHI negative control showed no killing ability as expected. However, there are also little Streptococcus Mutans growing near J23100+yebF+B0015 culture supernatant. By observing the pattern on the plate, we speculated that it might be the result of the diffusion of Ampicillin beside it.
UNIQa6064944afe08238-partinfo-00000001-QINU