Difference between revisions of "Part:BBa K1372000"

 
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<partinfo>BBa_K1372000 short</partinfo>
 
<partinfo>BBa_K1372000 short</partinfo>
  
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assembly of the BBa_J61051 biobrick (PSal/NahR biosensor) with the BBa_K228001 biobrick (SupD-tRNA). This construction is an intermediate in the BBa_K1372001 construction
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This BB is the result of the assembly of two BB:
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- BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time).
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- BBa_K228001(lien): for the transcription of SupD-tRNA.  
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[[File:Paris Saclay BB2000.png|center]]
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:08, 17 October 2014

salicylate inducible suppressing system

This BB is the result of the assembly of two BB: - BBa_J61051 (Lien vers la BB) for the expression of the protein NAHR, which binds to Sal promoter (Psal), to activate the transcription in response to the inducer: salicylate (substrate consumed by E. coli bacteria over time). - BBa_K228001(lien): for the transcription of SupD-tRNA.

Paris Saclay BB2000.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 786
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 77
    Illegal NgoMIV site found at 618
    Illegal AgeI site found at 1309
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1312