Difference between revisions of "Part:BBa K1462260"
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<partinfo>BBa_K1462260 short</partinfo> | <partinfo>BBa_K1462260 short</partinfo> | ||
| − | + | This part is constructed as a control group to BBa_K1462110 to identify Erg10 has been directed to the mitochondria. The GFP is fused at the C-terminus of Erg10 to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins. | |
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Revision as of 19:05, 17 October 2014
Gal1+cox6+Erg10+cyc1
This part is constructed as a control group to BBa_K1462110 to identify Erg10 has been directed to the mitochondria. The GFP is fused at the C-terminus of Erg10 to report where the enzyme located. Under the confocal microscope, we can observe the location of green fluorescence, thus to confirm the exact subcellular localization of target proteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1378
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000COMPATIBLE WITH RFC[1000]