Difference between revisions of "Part:BBa K1510005:Design"
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===Design Notes=== | ===Design Notes=== | ||
We used back insert to assemble two terminator together. There's no point mutation in this construct. In order to fit iGEM standard part, there are EcoR1 and Xba1 cutting sites in the front and Spe1 and Pst1 cutting sites in the last. | We used back insert to assemble two terminator together. There's no point mutation in this construct. In order to fit iGEM standard part, there are EcoR1 and Xba1 cutting sites in the front and Spe1 and Pst1 cutting sites in the last. | ||
| + | <p>[[File:NYMU_yenanchang_Electrophoresis_of_B0012+B0012.jpg ]]</p> | ||
| + | <p> electrophoresis of B0012+B0012 </p> | ||
Latest revision as of 18:57, 17 October 2014
B0012+B0012
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We used back insert to assemble two terminator together. There's no point mutation in this construct. In order to fit iGEM standard part, there are EcoR1 and Xba1 cutting sites in the front and Spe1 and Pst1 cutting sites in the last.
electrophoresis of B0012+B0012
Source
BBa_B0012+BBa_B0012(2014 iGEM kit)