Difference between revisions of "Part:BBa K1510002:Design"
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===Design Notes=== | ===Design Notes=== | ||
Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS. | Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS. | ||
| + | <p>[[File:NYMU_yenanchang_eletrophoration_fo_nlmC_promoter.jpg]]</p> | ||
| + | <p> electrophoration of nlmC promoter</p> | ||
Latest revision as of 18:42, 17 October 2014
PnlmC (S.mutans)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Since there is no accurate description of actual position of nlmC promoter in previous study, to ensure the function of this promoter, we amplified the sequence from two comE binding site( used for detecting CSP) to the last nucleotide before gene nlmC. As a result, the gene sequence consists of two comE binding site, nlmC promoter and RBS.
electrophoration of nlmC promoter
Source
Streptococcus mutans UA159
References
Liu, T., et al., ComCED signal loop precisely regulates nlmC expression in Streptococcus mutans. Annals of Microbiology, 2014. 64(1): p. 31-38.