Difference between revisions of "Part:BBa K1498000:Experience"
| Line 4: | Line 4: | ||
===Applications of BBa_K1498000=== | ===Applications of BBa_K1498000=== | ||
| + | |||
| + | Under the control of the constitutive promoter BBa_J23110 and following expression in ''E. coli'' BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of 36.5 kDa was visible, suggesting that the pre-processed protein was present. However only the processed form of the protein will exhibit activity. | ||
In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers). | In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers). | ||
| − | As a protease gelatin degredation should be observed. | + | As a protease gelatin degredation should also be observed. |
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:41, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1498000
Under the control of the constitutive promoter BBa_J23110 and following expression in E. coli BL21 the supernatant was analysed on a SDS-PAGE gel. Following coomassie staining a protein of 36.5 kDa was visible, suggesting that the pre-processed protein was present. However only the processed form of the protein will exhibit activity.
In theory BBa_K1498000 should be suitable to use in assays to degrade keratin rich structures (hair, nails, feathers).
As a protease gelatin degredation should also be observed.
User Reviews
UNIQ711bc6cfd39ab3c4-partinfo-00000000-QINU UNIQ711bc6cfd39ab3c4-partinfo-00000001-QINU