Difference between revisions of "Part:BBa K1431401:Design"
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Revision as of 18:39, 17 October 2014
One gRNA Sequence for HIV-1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Method
We used a method derived from the method described in the paper by Feng Zhang[http://www.nature.com/nbt/journal/v31/n9/abs/nbt.2647.html ZhangFgRNA].
As mentioned in main page:
Conserved Sequence Analysis
Strip out sequences without PAM
Select gRNA sequences with the best theoretical quality
Source
Conserved Region of the HIV-1 Genome from the NIH HIV-1 Sequence Database