Difference between revisions of "Part:BBa K1431401:Design"

(→‎Designing the Sequence)
Line 4: Line 4:
 
<partinfo>BBa_K1431401 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K1431401 SequenceAndFeatures</partinfo>
  
−
 
−
=== Designing the Sequence ===
 
  
 
We used a method derived from the method described in the paper by Feng Zhang<sup>[http://www.nature.com/nbt/journal/v31/n9/abs/nbt.2647.html ZhangFgRNA]</sup>.
 
We used a method derived from the method described in the paper by Feng Zhang<sup>[http://www.nature.com/nbt/journal/v31/n9/abs/nbt.2647.html ZhangFgRNA]</sup>.
 +
 +
As mentioned in main page:
 +
 +
Conserved Sequence Analysis
 +
 +
Strip out sequences without PAM
 +
 +
Select gRNA sequences with the best theoretical quality
  
 
===Source===
 
===Source===

Revision as of 18:39, 17 October 2014

One gRNA Sequence for HIV-1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


We used a method derived from the method described in the paper by Feng Zhang[http://www.nature.com/nbt/journal/v31/n9/abs/nbt.2647.html ZhangFgRNA].

As mentioned in main page:

Conserved Sequence Analysis

Strip out sequences without PAM

Select gRNA sequences with the best theoretical quality

Source

Conserved Region of the HIV-1 Genome from the NIH HIV-1 Sequence Database

References