Difference between revisions of "Part:BBa K1510010:Experience"
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===User Reviews=== | ===User Reviews=== | ||
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| − | To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by | + | To test if our yebF works, we fuse RFP at the C-terminal of the protein(YebF), and test if the signal protein works by measuring the fluorescence intensity in the culture supernatant. |
This is our result under Multimode microplate reader: | This is our result under Multimode microplate reader: | ||
[[File:NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg]] | [[File:NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg]] | ||
Latest revision as of 18:38, 17 October 2014
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1510010
Secreting red fluorescence protein (RFP) extracellularly, it can be used to test if YebF work. Anying circuit required secreted RFP can also make application.
User Reviews
UNIQ6e10880b090ce39b-partinfo-00000000-QINU
To test if our yebF works, we fuse RFP at the C-terminal of the protein(YebF), and test if the signal protein works by measuring the fluorescence intensity in the culture supernatant.
This is our result under Multimode microplate reader:
UNIQ6e10880b090ce39b-partinfo-00000001-QINU