Difference between revisions of "Part:BBa K1510010:Experience"

 
Line 1: Line 1:
 
 
__NOTOC__
 
__NOTOC__
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
 
This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
Line 5: Line 4:
  
 
===Applications of BBa_K1510010===
 
===Applications of BBa_K1510010===
 
+
Secreting RFP extracellularly, it can be used to test if YebF work. Anying circuit required secreted RFP can also make application.
 
===User Reviews===
 
===User Reviews===
 
<!-- DON'T DELETE --><partinfo>BBa_K1510010 StartReviews</partinfo>
 
<!-- DON'T DELETE --><partinfo>BBa_K1510010 StartReviews</partinfo>
 +
To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant.
 +
This is our result under Multimode microplate reader:
 +
[[File:NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg]]
 
<!-- Template for a user review
 
<!-- Template for a user review
 
{|width='80%' style='border:1px solid gray'
 
{|width='80%' style='border:1px solid gray'

Revision as of 18:36, 17 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1510010

Secreting RFP extracellularly, it can be used to test if YebF work. Anying circuit required secreted RFP can also make application.

User Reviews

UNIQe4d956a7b5bcbebc-partinfo-00000000-QINU To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant. This is our result under Multimode microplate reader: NYMU yebF fluroescence intensity of RFP in supernat partial enlargement detail dataJPEG.jpg UNIQe4d956a7b5bcbebc-partinfo-00000001-QINU