Difference between revisions of "Part:BBa K1321367"

 
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__NOTOC__
 
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<partinfo>BBa_K1321367 short</partinfo>
 
<partinfo>BBa_K1321367 short</partinfo>
  
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GATC
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A T7-promoter expression construct of super-folder GFP fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.
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This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]
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Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 18:33, 17 October 2014

sfGFP + linker-CBDcipA-linker with T7 promoter

A T7-promoter expression construct of super-folder GFP fused N-terminally to CBDcipA (a cellulose-binding domain), which contains an endogenous N and C-terminal linker sequence.

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: IC14-sfGFP-part-table.PNG

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 923
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 923
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 923
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 923
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 923
    Illegal NgoMIV site found at 53
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 68