Difference between revisions of "Part:BBa K1321366"

Latest revision as of 18:29, 17 October 2014

CBDcex fused to sfGFP with T7 promoter

A T7-promoter expression construct of super-folder GFP fused C-terminally to CBDcex (a cellulose-binding domain).

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below:

Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 53
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 401

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321366 short</partinfo>
-GATC
+A T7-promoter expression construct of super-folder GFP fused C-terminally to CBDcex (a cellulose-binding domain).
+This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]
+Note that the stop codon plus 6 bp at the end of the sequence are included the RFC25 suffix which is not shown. The prefix to this part is RFC10 format.
 <!-- Add more about the biology of this part here