Difference between revisions of "Part:BBa K1498001:Design"
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===Source=== | ===Source=== | ||
| − | Sourced from the BioBricks website, BBa_K258006 was synthesised as it was not available from the catalogue | + | Sourced from the BioBricks website, BBa_K258006 was synthesised as it was not available from the catalogue (but codon optimised for use in ''E. coli'') |
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| + | BBa_J23110 was retrieved from the distribution kit | ||
===References=== | ===References=== | ||
Chung, C. W., You, J., Kim, K., Moon, Y., Kim, H., & Ahn, J. H. (2009). Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD). Microbial cell factories, 8(1), 11. doi:10.1186/1475-2859-8-11 | Chung, C. W., You, J., Kim, K., Moon, Y., Kim, H., & Ahn, J. H. (2009). Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD). Microbial cell factories, 8(1), 11. doi:10.1186/1475-2859-8-11 | ||
Latest revision as of 17:59, 17 October 2014
constitutive promoter with lipase (BBa_J23110, BBa_K258006)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 692
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Codon optimised for E. coli.
Source
Sourced from the BioBricks website, BBa_K258006 was synthesised as it was not available from the catalogue (but codon optimised for use in E. coli)
BBa_J23110 was retrieved from the distribution kit
References
Chung, C. W., You, J., Kim, K., Moon, Y., Kim, H., & Ahn, J. H. (2009). Export of recombinant proteins in Escherichia coli using ABC transporter with an attached lipase ABC transporter recognition domain (LARD). Microbial cell factories, 8(1), 11. doi:10.1186/1475-2859-8-11