Difference between revisions of "Part:BBa K1321346"

Revision as of 17:41, 17 October 2014

CBDclos fused to sfGFP in RFC 25

super-folder GFP fused C-terminally to CBDclos (a cellulose-binding domain), which contains an endogenous N-terminal linker sequence.

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 316

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 __NOTOC__
 <partinfo>BBa_K1321346 short</partinfo>
-asdf
+super-folder GFP fused C-terminally to CBDclos (a cellulose-binding domain), which contains an endogenous N-terminal linker sequence.
+This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]
+Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
 <!-- Add more about the biology of this part here