Difference between revisions of "Part:BBa K1321342"

Revision as of 17:38, 17 October 2014

sfGFP fused to CBDcex in RFC 25

sfGFP fused N-terminally to CBDcex (a cellulose-binding domain).

This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 10

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1321342 short</partinfo>
-A LacI-promoter expression construct of sfGFP fused N-terminally to CBDcex (a cellulose-binding domain).
+sfGFP fused N-terminally to CBDcex (a cellulose-binding domain).
 This construct is part of a library of Super-folder GFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]