Difference between revisions of "Part:BBa K1321341"

Revision as of 17:28, 17 October 2014

sfGFP fused to CBDclos in RFC 25

Super-folder GFP fused N-terminally to CBDclos (a cellulose-binding domain).

This construct is part of a library of sfGFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below:

Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 10

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1321341 short</partinfo>
-asdfads
+Super-folder GFP fused N-terminally to CBDclos (a cellulose-binding domain).
+This construct is part of a library of sfGFP fusions with cellulose binding domains, which we used to assay the CBD binding affinity. Please see our [http://2014.igem.org/Team:Imperial/Functionalisation project page] for more information. The collection of sfGFP-CBD fusion parts can be seen in the table below: [[File:IC14-sfGFP-part-table.PNG]]
+Note that the start and stop codon, plus 6 bp either side of the sequence, are included the RFC25 prefix and suffix which is not shown.
 <!-- Add more about the biology of this part here