Difference between revisions of "Part:BBa K1510009:Experience"

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===User Reviews===
 
===User Reviews===
 
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To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant.
 
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Revision as of 17:23, 17 October 2014

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1510009

Using yebF at the N-terminal of desired sequence as recombinant protein would help exporting it extracellularly. Especially useful when common experimental strains, such as K-12 are used, since they don't naturally secrete protein outside.

User Reviews

UNIQe664246144c37618-partinfo-00000000-QINU To test if our yebF works, we fuse RFP at the C-terminal of the protein, and test if the signal protein works by examing the fluorescence intensity in the culture supernatant. UNIQe664246144c37618-partinfo-00000001-QINU