Difference between revisions of "Part:BBa K1462000"
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CoxVI is short for the subunit VI of the yeast cytochrome c oxidase, a N-terminal signal peptide to mitochondrial matrix. | CoxVI is short for the subunit VI of the yeast cytochrome c oxidase, a N-terminal signal peptide to mitochondrial matrix. | ||
| − | Most mitochondrial proteins are synthesized in the cytosol as larger precursors carrying mitochondria targeting signals. In this pathway, the precursor is bound by cytosolic chaperones and then delivered to a set of receptors on the outer surface of mitochondria. Then, the polypeptide chain is passed through the TOM complex in the outer membrane and the TIM23 complex in the inner membrane. Insertion into the inner membrane is driven electrophoretically by the electrochemical potential across the membrane. Finally, the precursor is pulled completely across the membrane into the matrix by an ATP-powered translocation motor attached to the inner side of the TIM23complex. After that, the protein will be refolded by mitochondrial chaperone, and the mitochondria targeting signal will be cleaved.( | + | Most mitochondrial proteins are synthesized in the cytosol as larger precursors carrying mitochondria targeting signals. In this pathway, the precursor is bound by cytosolic chaperones and then delivered to a set of receptors on the outer surface of mitochondria. Then, the polypeptide chain is passed through the TOM complex in the outer membrane and the TIM23 complex in the inner membrane. Insertion into the inner membrane is driven electrophoretically by the electrochemical potential across the membrane. Finally, the precursor is pulled completely across the membrane into the matrix by an ATP-powered translocation motor attached to the inner side of the TIM23complex. After that, the protein will be refolded by mitochondrial chaperone, and the mitochondria targeting signal will be cleaved.(Fig.3) |
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| + | [[File:COX6 leading peptide mechanism.jpg|600px|center|thumb|''Fig.3:'' COX VI LEADING PEPTIDE MECHANISM]] | ||
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Revision as of 16:56, 17 October 2014
CoxVI
CoxVI is short for the subunit VI of the yeast cytochrome c oxidase, a N-terminal signal peptide to mitochondrial matrix.
Most mitochondrial proteins are synthesized in the cytosol as larger precursors carrying mitochondria targeting signals. In this pathway, the precursor is bound by cytosolic chaperones and then delivered to a set of receptors on the outer surface of mitochondria. Then, the polypeptide chain is passed through the TOM complex in the outer membrane and the TIM23 complex in the inner membrane. Insertion into the inner membrane is driven electrophoretically by the electrochemical potential across the membrane. Finally, the precursor is pulled completely across the membrane into the matrix by an ATP-powered translocation motor attached to the inner side of the TIM23complex. After that, the protein will be refolded by mitochondrial chaperone, and the mitochondria targeting signal will be cleaved.(Fig.3)
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]