Difference between revisions of "Part:BBa K1510009:Design"

 
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<partinfo>BBa_K1510009 short</partinfo>
 
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===References===
 
===References===
 +
*(2006)"Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli"
 +
*(2007)"ACES™ Signal Sequence and YebF Expression Systems Technical Brief"
 +
*(2012)"A Protein Export Pathway Involving Escherichia coli Porins"
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*(2006)patent" PROTEIN PRODUCTION METHOD UTILIZING YEBF "

Revision as of 16:46, 17 October 2014

E.coli Signal sequence YebF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 159
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

To fit igem part standard, the circuit is constructed in backbone pSB1C3, chloramphenicol with EcoR1 and Xba1 in front of the part, and Spe1, Pst1 behind the part.


Source

The promoter, J23100, comes from the 2014 distributed igem kit. The signal sequence, yebF, encoding signal protein exporting recombinant protein extracellular, is from E.coli K-12. By designing primer, we obtain it together with its naturally occurring ribosomal binding site using PCR and extraction. Finally, the terminator B0015 is from 2014 igem distribution.

References

  • (2006)"Extracellular accumulation of recombinant proteins fused to the carrier protein YebF in Escherichia coli"
  • (2007)"ACES™ Signal Sequence and YebF Expression Systems Technical Brief"
  • (2012)"A Protein Export Pathway Involving Escherichia coli Porins"
  • (2006)patent" PROTEIN PRODUCTION METHOD UTILIZING YEBF "