Difference between revisions of "Part:BBa K1453005"

 
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<partinfo>BBa_K1453005 short</partinfo>
 
<partinfo>BBa_K1453005 short</partinfo>
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This part is a first kind of connectee, which we used to check the connection between connectee and connector in our basic test.
 
This part is a first kind of connectee, which we used to check the connection between connectee and connector in our basic test.
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== Structure ==
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https://static.igem.org/mediawiki/2014/4/41/Membrane_TAL1.png
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The structure is based on the BBa_1453000 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.  
 
The structure is based on the BBa_1453000 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.  
  

Revision as of 16:24, 17 October 2014

ssDsbA-mRFP-Lgt-TAL1-His Tag This part is a first kind of connectee, which we used to check the connection between connectee and connector in our basic test.

Structure

Membrane_TAL1.png

The structure is based on the BBa_1453000 and the recognition sequence is T-TCGATATCAAGC-T. Therefore, the TAL-Protein DiRepeats protein we need are BBa_K747013, BBa_K747024, BBa_K747044, BBa_K747061, BBa_K747064 and BBa_K747089.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 612
    Illegal AgeI site found at 724
    Illegal AgeI site found at 1537
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1158
    Illegal BsaI.rc site found at 2176
    Illegal BsaI.rc site found at 2788