Difference between revisions of "Part:BBa K1362054:Design"
 
Line 13: Line 13:
 
===Source===
 
===Source===
 
C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli.  
 
C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli.  
−
The DNA sequence of the SsrA tag with overhangs was ordered as a DNA oligo and cloned into  (<partinfo>BBa_K1362052</partinfo>).
+
The DNA sequence of the SsrA tag with overhangs was ordered as DNA oligos and cloned into  (<partinfo>BBa_K1362052</partinfo>).
  
  
 
===References===
 
===References===

Latest revision as of 16:24, 17 October 2014

SsrA degradation tag as RFC[105] C-terminal insert


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 67


Design Notes

Source

C-terminal degradation tags are commonly found in high turnover proteins in Escherichia coli. The DNA sequence of the SsrA tag with overhangs was ordered as DNA oligos and cloned into (BBa_K1362052).


References