Difference between revisions of "Part:BBa K1362255:Design"
 
Line 12: Line 12:
 
===Source===
 
===Source===
 
origin: Bacillus subtilis <br />
 
origin: Bacillus subtilis <br />
−
The part sequence as described by Szeto et al. [[#References|[1]]] was synthesized.
+
The part sequence as described by Szeto et al. [[#References|[1]]] was ordered as DNA oligos.
  
  

Latest revision as of 16:19, 17 October 2014

MinD MTS tag


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 67
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 16


Design Notes

C-terminal tag
Note: There are no stop codons included in this sequence.

Source

origin: Bacillus subtilis
The part sequence as described by Szeto et al. [1] was ordered as DNA oligos.


References

[1] Tim H. Szeto et al. Membrane localization of MinD is mediated by a C-terminal motif that is conserved acrosseubacteria, archaea, and chloroplasts PNAS 2002 99 (24) 15693-15698 Published online: 22 May 2005 | doi:10.1073/pnas.232590599