Difference between revisions of "Part:BBa K1362055:Design"
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===Source=== | ===Source=== | ||
origin: Bacillus subtilis <br /> | origin: Bacillus subtilis <br /> | ||
| − | The part sequence as described by Szeto et al. [[#References|[1]]] was | + | The part sequence as described by Szeto et al. [[#References|[1]]] was ordered as DNA oligos. |
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===References=== | ===References=== | ||
[1] Tim H. Szeto et al.: Membrane localization of MinD is mediated by a C-terminal motif that is conserved acrosseubacteria, archaea, and chloroplasts. PNAS 2002 99 (24) 15693-15698. Published online: 22 May 2005 | doi:10.1073/pnas.232590599. | [1] Tim H. Szeto et al.: Membrane localization of MinD is mediated by a C-terminal motif that is conserved acrosseubacteria, archaea, and chloroplasts. PNAS 2002 99 (24) 15693-15698. Published online: 22 May 2005 | doi:10.1073/pnas.232590599. | ||
Latest revision as of 16:18, 17 October 2014
MinD MTS as RFC[105] C-terminal insert
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 95
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 106
Illegal SapI.rc site found at 44
Design Notes
Source
origin: Bacillus subtilis
The part sequence as described by Szeto et al. [1] was ordered as DNA oligos.
References
[1] Tim H. Szeto et al.: Membrane localization of MinD is mediated by a C-terminal motif that is conserved acrosseubacteria, archaea, and chloroplasts. PNAS 2002 99 (24) 15693-15698. Published online: 22 May 2005 | doi:10.1073/pnas.232590599.