Difference between revisions of "Part:BBa K1362051:Design"
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===Source=== | ===Source=== | ||
organism: Synechocystis species | organism: Synechocystis species | ||
| + | The intein sequence was kindly provided by Prof. Dr, Henning Motz from Westfälische Wilhelms-University Münster. | ||
Latest revision as of 16:12, 17 October 2014
Intein protease with arabinose inducible regulatory promoter/ repressor araC-Pbad-T7RBS-His-SspDnaBC
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1144
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 979
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 961
Design Notes
Source
organism: Synechocystis species The intein sequence was kindly provided by Prof. Dr, Henning Motz from Westfälische Wilhelms-University Münster.
References
Volkmann, G. et al: Controllable protein cleavages through intein fragment complementation. Protein Sci, 18 (2009), pp. 2393–2402. DOI: 10.1002/pro.249.