Difference between revisions of "Part:BBa K1328000"
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HXT1-CMVmini | HXT1-CMVmini | ||
https://static.igem.org/mediawiki/2014/4/40/2014-HXT1%2BCMV-forget.png | https://static.igem.org/mediawiki/2014/4/40/2014-HXT1%2BCMV-forget.png | ||
| − | Part HXT1+CMV is used as promoter for target gene under the regulation of glucose, consisting of HXT-1 promoter and CMV promoter. HXT-1 is a low-affinity glucose transporter from Saccharomyces cerevisiae. There is a glucose response element in HXT-1 gene sensitive to glucose in human fibroblasts, which is used here as a glucose-dependent regulator. | + | |
| + | Part HXT1+CMV is used as promoter for target gene under the regulation of glucose, consisting of HXT-1 promoter and CMV promoter. | ||
| + | HXT-1 is a low-affinity glucose transporter from Saccharomyces cerevisiae. There is a glucose response element in HXT-1 gene sensitive to glucose in human fibroblasts, which is used here as a glucose-dependent regulator. | ||
| + | Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose. | ||
Revision as of 15:49, 17 October 2014
HXT1-CMVmini
Part HXT1+CMV is used as promoter for target gene under the regulation of glucose, consisting of HXT-1 promoter and CMV promoter. HXT-1 is a low-affinity glucose transporter from Saccharomyces cerevisiae. There is a glucose response element in HXT-1 gene sensitive to glucose in human fibroblasts, which is used here as a glucose-dependent regulator. Expression of target gene under the control of HXT1-CMV promoter is induced by high concentration of glucose.