Difference between revisions of "Part:BBa K1331001"

Revision as of 15:11, 17 October 2014

Modified rhlA coding sequence from Pseudomonas aeruginosa SQ6

This is an improvement of Part:BBa_K317998 designed by iGEM10_Tokyo-NoKoGen. A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence. The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.

RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in Pseudomonas aeruginosa.This part encodes rhamnosyltransferase I subunit A, and the other part Part:BBa_K1331004 encodes subunit B.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 69
Illegal BamHI site found at 629
Illegal XhoI site found at 805
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 294
Illegal BsaI.rc site found at 478

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K1331001 short</partinfo>
 This is an improvement of [https://parts.igem.org/Part:BBa_K317998 Part:BBa_K317998] designed by iGEM10_Tokyo-NoKoGen.
 A mutation has been done to remove the PstI restriction site (at 720) to meet BioBrick RFC[10] requirement without changing the amino acid sequence. The sequence we provide here includes the whole coding sequence and a part of downstream noncoding sequence of rhlA.
-RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.This part encodes rhamnosyltransferase I subunit A, and the other part [https://parts.igem.org/Part:BBa_K1331004 rhlB] encodes subunit B.
+RhlA together with rhlB encodes rhamnosyltransferase I for mono-rhamnolipids biosynthesis in ''Pseudomonas aeruginosa''.This part encodes rhamnosyltransferase I subunit A, and the other part [https://parts.igem.org/Part:BBa_K1331004 Part:BBa_K1331004] encodes subunit B.
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