Difference between revisions of "Part:BBa B0011"

Line 8: Line 8:
 
===Usage and Biology===
 
===Usage and Biology===
 
Appears to be a bidirectional terminator since it contains a string of T's on the direct strand after the stem loop and on the reverse strand before the
 
Appears to be a bidirectional terminator since it contains a string of T's on the direct strand after the stem loop and on the reverse strand before the
stem loop. It is also found between two coding regions that point towards each other.
+
stem loop. Also, it is found between two coding regions that point towards each other.
  
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 11:52, 26 April 2006

Designed by Reshma Shetty Entered: 2003-01-31

LuxICDABEG (+/-)

Bidirectional transcriptional terminator consisting of a 22bp stem-loop.

Usage and Biology

Appears to be a bidirectional terminator since it contains a string of T's on the direct strand after the stem loop and on the reverse strand before the stem loop. Also, it is found between two coding regions that point towards each other.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

Functional Parameters

biologyLuxIA
directionBidirectional
forward_efficiency0.419[CC]/0.95[JK]
n/aForward
reversed_version585
reverse_efficiency0.636[CC]/0.86[JK]