Difference between revisions of "Part:BBa K1493601"

Revision as of 13:25, 17 October 2014

Kis Antitoxin (Kis-Kid Toxin-Antitoxin System)

Kis (antitoxin) and Kid (toxin) are part of the parD stability system of plasmid R1 acting as a pre-segregation mechanism to ensure plasmid proliferation. Kid breaks down single stranded messenger RNAs. By degradation of something as vital as messenger RNA, this toxin does not differ in function with gram positive and gram negative bacteria. In nature, Kid causes cells to divide slower to ensure that every daughter cell receives the R1 plasmid. The Kis-Kid interaction has proven to be a 1:2 ratio [1, 2].

The Kis antitoxin was amplified and elongated form the pAB24 plasmid.

iGEM Wageningen team 2014 acchieved cloning of Kid (Toxin) in a low copy number plasmid (psB3K3) in combination with the antitoxin, if a glycerol stock

For more information of all the system we design and steps we follow, visit the <a href="http://2014.igem.org/http://2014.igem.org/Team:Wageningen_UR/project/gene_transfer" class="soft_link">iGEM Wageningen team 2014 wiki </a>.

References:

1. de la Cueva-Méndez, G. and B. Pimentel, Gene and cell survival: lessons from prokaryotic plasmid R1. EMBO reports, 2007. 8(5): p. 458-464. 2. Ruiz-Echevarría, M.J., et al., Kid, a small protein of the parD stability system of plasmid R1, is an inhibitor of DNA replication acting at the initiation of DNA synthesis. Journal of molecular biology, 1995. 247(4): p. 568-577.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 131
1000
COMPATIBLE WITH RFC[1000]

Functional Parameters

References

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1493601 short</partinfo>
-Kis (antitoxin) and Kid (toxin) are part of the parD stability system of plasmid R1 acting as a pre-segregation mechanism to ensure plasmid proliferation. Kid breaks down single stranded messenger RNAs. By degradation of something as vital as messenger RNA, this toxin does not differ in function with gram positive and gram negative bacteria. In nature, Kid causes cells to divide slower to ensure that every daughter cell receives the R1 plasmid. In our system an excess of Kid will result in a bactericidal effect. Additionally, the Kis-Kid interaction has proven to be a 1:2 ratio [5, 6].
+Kis (antitoxin) and Kid (toxin) are part of the parD stability system of plasmid R1 acting as a pre-segregation mechanism to ensure plasmid proliferation. Kid breaks down single stranded messenger RNAs. By degradation of something as vital as messenger RNA, this toxin does not differ in function with gram positive and gram negative bacteria. In nature, Kid causes cells to divide slower to ensure that every daughter cell receives the R1 plasmid. The Kis-Kid interaction has proven to be a 1:2 ratio [1, 2].
+The Kis antitoxin was amplified and elongated form the pAB24 plasmid.
+iGEM Wageningen team 2014 acchieved cloning of Kid (Toxin) in a low copy number plasmid (psB3K3) in combination with the antitoxin, if a glycerol stock <i> </i>
+For more information of all the system we design and steps we follow, visit the <a href="http://2014.igem.org/http://2014.igem.org/Team:Wageningen_UR/project/gene_transfer"  class="soft_link">iGEM Wageningen team 2014 wiki </a>.
+<b>References:</b>
+. de la Cueva-Méndez, G. and B. Pimentel, Gene and cell survival: lessons from prokaryotic plasmid R1. EMBO reports, 2007. 8(5): p. 458-464.
+. Ruiz-Echevarría, M.J., et al., Kid, a small protein of the parD stability system of plasmid R1, is an inhibitor of DNA replication acting at the initiation of DNA synthesis. Journal of molecular biology, 1995. 247(4): p. 568-577.
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