Difference between revisions of "Part:BBa K1415208"

Revision as of 12:54, 17 October 2014

Pcons+B0034+PBAN(Solenopsis invicta)+B0034+BFP+J61048

To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.

Fig.1-1 Biobrick of Pcons + RBS + PBAN(SI) + BFP + Term.

Fig.1-2 The bluelight test of our E.coli

Fig.1-3 Modeling result of Pcons + RBS + PBAN(SI) + BFP + Ter. The blue line is the expression profile of the theoretical biobrick. And the green line is the expression data of Pcons + RBS + PBAN(SI) + BFP + Ter. And the red line is the adjusting line from the green and blue one. This line represent the correcting line of theoretical data and real condition data which can make our model not only fit the theoretical condition but also stay away from experimental bias.

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 76
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 888
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K1415208 short</partinfo>
-We use this part as reporter gene, it can check whether our PBAN works and quantify its expression.
+To predict the PBAN expression in E.coli by computer modeling, we next tested PBAN BFP biobricks. We obtained the average expressive value of the blue fluorescence in the biobrick part (above) and also the control part of Pcons + RBS + BFP + Ter. Therefore, we can use the average value to generate predictions of the PBAN expression in E.coli.
 [[File:ALLSI.png|thumb|left|900px|'''Fig.1-1''' Biobrick of Pcons + RBS + PBAN(SI) + BFP + Term.]]
 [[File:BLAI.png|thumb|left|900px|'''Fig.1-2'''  The bluelight test of our E.coli]]