Difference between revisions of "Part:BBa K1398003"
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The Exeter 2014 team also showed that NemA cannot perform this catalytic function without cofactors: FMN, NADH and NADPH. | The Exeter 2014 team also showed that NemA cannot perform this catalytic function without cofactors: FMN, NADH and NADPH. | ||
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Full article at: http://2014.igem.org/Team:Exeter/EnzymeValidation | Full article at: http://2014.igem.org/Team:Exeter/EnzymeValidation | ||
Revision as of 12:44, 17 October 2014
NemA (Inducible Construct)
A construct created by the 2014 Exeter iGEM team to degrade TNT and nitroglycerin. The construct contains the coding sequence for NemA (BBa_K1398002), an enzyme involved in the degradation of toxic compounds for their reuse in nitrogen metabolism. The construct also contains a Lactose-inducible promoter (BBa_R0010 ), a strong RBS (BBa_B0034) and a double terminator made up of BBa_B0010 and BBa_B0012. The protein has been codon-optimised for expression in E. coli.
The Exeter 2014 team used Raman spectroscopy to show the breakdown of Nitroglycerin by NemA:
The Exeter 2014 team also showed that NemA cannot perform this catalytic function without cofactors: FMN, NADH and NADPH.
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Full article at: http://2014.igem.org/Team:Exeter/EnzymeValidation
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 746
Illegal AgeI site found at 583 - 1000COMPATIBLE WITH RFC[1000]